What is an ELISA?

ELISA (Source: Wikimedia)
ELISA (Source: Wikimedia)

ELISA is the short form for enzyme-linked immunosorbent assay. It is a plate-based assay technique. In other words, it is a serological method in which serum and other body fluids are studied. ELISA is designed to detect and quantify soluble substances (a.l.*), such as proteins, peptides, antibodies, and hormones.

The process of ELISA consists of immobilization of antigen (target molecule) on a microplate, and its complexation with antibody. This antibody-antigen interaction is very important process. Nevertheless, the after complexation with antibody, the process of linking to reporter enzyme takes place, then the incubation with appropriate substrate occurs, and eventually, there is an enzyme reaction and production of measurable product. Consequently, measurement of the activity of the reporter enzyme helps in detection and quantification.

Zusammenfassung in deutscher Sprache (Summary in German language)

ELISA ist enzyme-linked immunosorbent assay. Es ist eine serologische Methode zur Untersuchung löslicher Substanzen wie Proteine, Peptide, Antikörper, und Hormone. In diesem Prozess interagiert das Antigen mit dem Antikörper und verbindet sich mit dem Enzym. Ein geeignetes Substrat wird hinzugefügt. Die Aktivität des Enzyms hilft beim Nachweis und bei der Quantifizierung.

For further information:

Enzyme-linked Immunosorbent Assay (ELISA): From A to Z – https://amzn.to/388le7H (a.l.*)

Advances in Molecular Techniques – https://amzn.to/388xhln (a.l.*)

The 2021-2026 World Outlook for Enzyme-Linked Immunosorbent Assay (ELISA) Technologies – https://amzn.to/2NBZ4Bb (a.l.*)

*a.l. = affiliate link

Usman Zafar Paracha

Usman Zafar Paracha is a sort of entrepreneur. He is the author of "Color Atlas of Statistics", and the owner of an Android game "Faily Rocket."