It is a homologous recombination-mediated genetic engineering to create genetic constructs. This process does not require the use of restriction enzymes and DNA ligase. It uses bacterium’s homologous recombination and it is beneficial in recombination of DNA without the traditional molecular biology technique of “cutting and pasting.”
Recombineering provides the ability to make genetic alterations to any DNA sequence in a chromosome or cloned onto a vector replicating in recombineering-proficient bacteria, such as E. coli. This process can create genetic constructs that could not be made with in vitro genetic engineering. Interestingly, this process occurs in a precise, rapid, inexpensive, and efficient manner.
DNA ligase: Enzyme that connect two DNA strands.
Homologous recombination: A process of the exchange of genetic information between two DNA strands.
Restriction enzymes: Enzymes that cut DNA at target sequences.
Summary in German language (Zusammenfassung in deutscher Sprache)
Recombineering ist eine homologe rekombinationsvermittelte Gentechnik zur Erzeugung genetischer Konstrukte. In der Gentechnik ist dies eine präzise, schnelle, kostengünstige und effiziente Methode.
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